Search results for "enzymatic stability"

showing 4 items of 4 documents

Potential dopamine prodrug-loaded liposomes: preparation, characterization, andin vitrostability studies

2010

Dopamine delivery to the central nervous system (CNS) undergoes the permeability limitations of the blood-brain barrier (BBB). Condensation of dopamine with neutral amino acids could afford potential pro- drugs able to interact with the BBB endogenous transporters and easily enter the brain. To improve the bio-availability of the dopamine prodrug, 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (DOPH), it was encapsulated in unilamellar liposomes of dimiristoylphosphatidylcholine (DMPC)and cholesterol. Vesicles were characterized by dynamic light scattering in order to evaluate their dimensions and vesicle stability, by zeta-potential measurements, by means of electronic mi…

LightDopaminePhenylalanineenzymatic stabilityPharmaceutical Sciencechemical stability; dopamine prodrug; enzymatic stability; liposomeschemical stabilityDrug StabilityDynamic light scatteringDopamineZeta potentialmedicineHumansScattering RadiationProdrugsLiposomeChromatographyCalorimetry Differential ScanningChemistryVesicleProdrugSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoPermeability (electromagnetism)LiposomesliposomeBiophysicsChemical stabilityDimyristoylphosphatidylcholineDopamine prodrugmedicine.drugJournal of Liposome Research
researchProduct

Synthesis and in vitro studies on a potential dopamine prodrug.

2008

Dopamine delivery to the central nervous system (CNS) undergoes the permeability limitations of blood-brain barrier (BBB) which is a selective interface that excludes most water-soluble molecules from entering the brain. Neutral amino acids permeate the BBB by specific transport systems. Conden- sation of dopamine with neutral amino acids could afford potential prodrugs able to interact with the BBB endogenous transporters and easily enter the brain. The synthesis and characterization of the dopamine derivative 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (7) is de- scribed. The chemical and enzymatic stability of 7 was evaluated. The molecular weight (300 Da) and Log P …

Magnetic Resonance SpectroscopyChemistry PhysicalDopamineenzymatic stabilityMembranes ArtificialBuffersSettore CHIM/08 - Chimica FarmaceuticaRatschemical stabilityRats Sprague-DawleyDrug Delivery SystemsSolubilitySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDopamine ProdrugAnimalsHumansIndicators and ReagentsProdrugsIntestinal MucosaDie Pharmazie
researchProduct

N-Valproyl-L-Phenylalanine as new potential antiepileptic drug: Synthesis, characterization and in vitro studies on stability, toxicity and anticonvu…

2013

Valproic acid (VPA) is considered first-line drug in treatment of generalized idiopathic seizures such as absence, generalized tonic-clonic and myoclonic seizures. Among major antiepileptic drugs, VPA is also considered effective in childhood epilepsies and infantile spasms. Due to its broad activity, VPA acts as a mood stabilizer in bipolar disorder and it is useful in migraine prophylaxis. Despite its long-standing usage, severe reactions to VPA, such as liver toxicity and teratogenicity, are reported. To circumvent side effects due to structural characteristics of VPA, we synthesized in good yield a new VPA-aminoacid conjugate, the N-valproyl-L-Phenylalanine, and characterized by FT-IR, …

MaleDrugCell Membrane PermeabilityAminoacidic derivative Astrocytes toxicity CNS-Targeting Enzymatic Stability Hippocampal epilepsy Valproic acid.Cell Survivalmedicine.drug_classPhenylalaninemedicine.medical_treatmentmedia_common.quotation_subjectPrimary Cell CulturePhenylalaninePharmacologySettore BIO/09 - FisiologiaHippocampusTissue Culture TechniquesDrug StabilityDrug DiscoverymedicineAnimalsRats WistarEvoked Potentialsmedia_commonValproic AcidChemistryHydrolysisValproic AcidBiological TransportMood stabilizerMicrotomyHydrogen-Ion ConcentrationIn vitroRatsAnticonvulsantSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoAstrocytesToxicityAnticonvulsantslipids (amino acids peptides and proteins)Conjugatemedicine.drug
researchProduct

N-valproyl-L-tryptophan for CNS-targeting: synthesis, characterization and efficacy in vitro studies of a new potential antiepileptic drug.

2010

A new aminoacidic derivative of valproic acid (VPA) has been synthesized and characterized by analytical and spectral data. The rationale for the preparation of such potential antiepileptic agent is based on the observation that chemical combination of the anticonvulsant pharmacophore, VPA with essential aminoacids could afford more effective and less toxic actives. The synthesis, characterization, physico-chemical parameters functional for crossing Blood Brain Barrier of N-valproyl-L-tryptophan (4) are reported. The Log D pH7.4 (0.3) indicates that (4) is adequate to cross biological membranes. Its chemical and enzymatic stability were assessed. The experiments indicate high stability of c…

Malemedicine.medical_treatmentHippocampal formationPharmacologyIn Vitro TechniquesBlood–brain barrierSettore BIO/09 - FisiologiaHippocampuschemistry.chemical_compoundDrug StabilityIn vivoDrug DiscoverymedicineAnimalsRats WistarValproic AcidEpilepsyDipeptidesAminoacidic derivative Antiepileptic Drug CNS-Targeting Enzymatic Stability Seizure Like Events Model Valproic acidIn vitroElectrophysiological PhenomenaRatsmedicine.anatomical_structureAnticonvulsantchemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoAnticonvulsantsPharmacophoreDerivative (chemistry)medicine.drugMedicinal chemistry (Shariqah (United Arab Emirates))
researchProduct